The SMCL NGS Hub currently operates Illumina MiSeq (x3), NextSeq & HiSeq 4000 sequencing platforms.
We can incorporate sequencing into library preparation workflows or as a sequencing only service.
For sequencing only services, we would request detailed information on the library preparation, QC and quantification results (this can be provided for an additional charge), platform to be used, and desired sequencing required (advise can be given on this).
MiSeq
Suitable for targeted & small-genome sequencing, with quick run times. Available in two chemistry types, with multiple read lengths kits available
- v2 Chemistry
- 50 (S), 300 (S, M & N) & 500 (S & N) cycle
- Standard (S) = 15M reads
- Micro (M) = 4M reads
- Nano (N) = 1M reads
- Max read length 2x250bp
- v3 Chemistry
- 150 & 600 cycle
- Max read length 2x300bp
NextSeq 500
Suitable for larger scale sequencing (e.g. large panel & transcriptomics), where speed and flexibility are required. Available in two output types, with read length flexibility
- Mid Output = 160M reads
- 150 cycle
- 300 cycle
- High Output = 400M reads
- 75 cycle
- 150 cycle
- 300 cycle
HiSeq 4000
Suitable for large scale projects that require high data output (e.g. whole transcriptome & whole exomes). Can generate upwards of 312M reads per lane, or 2.5 Billion per flowcell (across 8 lanes). Various read lengths available; 50, 150 & 300 cycle kits available (or variations of up to max of 2x150bp). Sequencing on the HiSeq 4000 can be purchased as complete flow cells (i.e 8 lanes) or on a lane by lane basis (subject to current availability and throughput).
*Individual access will be granted on a project by project basis
